Evaluation of antioxidant activity of ethanol extract of roots obtained from asparagus racemosus by in vitro and ex vivo models

M. Kundu1*, R. Mazumder2, M. D. Kushwaha3

1 Govt. Polytechnic Uttarkashi, Dist:Uttarkashi, Uttarakhand, 249193

2 Department of Pharmaceutical Technology, Noida Institute of Engineering and Technology, Greater Noida, Uttar Pradesh 201306

3 Govt.P.G.College Uttarkashi, Dist: Uttarkashi, Uttarakhnad

Running Title: Antioxidant activity of Asparagus racemosus roots

*Corresponding author:

Email: mintu_phytochem@yahoo.co.in

ABSTRACT

Asparagus racemosus Willd . (Family: Liliaceae) commonly known as satawar, satavari or shatavari, is a tall climber, undershrub, distributed in low jungles of tropical and subtropical parts of India, Ceylon, Africa, Java and Ausrtalia . The roots of the plant has been used as antidiarrhoeal, appetizer, stomachic, expectorant, laxative, tonic, inflammations biliousness, useful in dysentery, diuretic. The plant contains a number of secondary metabolites namely sterols and saponins. From extensive literature survey it was revealed that no reports were available on in vitro and ex vivo antioxidant activity of Asparagus racemosus. The objective of the study was to explore the antioxidant activity of ethanol extract of roots of Asparagus racemosus by in vitro and ex vivo models to substantiate the folklore claim of the traditional practitioners. The roots of the plant showed sufficient antioxidant activity by in vitro (determination of total antioxidant activity through estimation of the conjugated dienes and thiobarbituric acid reactive substances formed, total phenolic content, reducing power, free radical scavenging activity by DPPH method, nitric oxide scavenging activity, hydroxyl radical scavenging activity and super oxide scavenging activity by NBT and DMSO methods) and ex vivo (inhibition of haemolysis of RBC induced by phenyl hydrazine, inhibition of lipid peroxidation induced by ferrous sulphate and carbon tetrachloride) models. The experimental data were compared with that of standard antioxidant like ascorbic acid, alpha-tocopherol acetate, butylated hydroxyl anisol (BHA) and butylated hydroxyl toluene (BHT). All observed results indicated ethanol extract of roots of Asparagus racemosus to possess antioxidant activity in a concentration dependant manner and the activity of the extract was found to be very much comparable to that of the selected standard drugs.

Keywords: Asparagus racemosus , Liliaceae, In-vitro and Ex-vivo Antioxidant, Ethanol extract, Roots.

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