Evaluation of antioxidant activity of ethanol extract of roots obtained
from Asparagus racemosus by in vitro and ex vivo models
M. Kundu1*, R. Mazumder2, M. D. Kushwaha 3
1
Govt. Polytechnic Uttarkashi, Dist:Uttarkashi, Uttarakhand 249193,
India.
2
Department of Pharmaceutical Technology, Noida Institute of Engineering
and Technology, Greater Noida, Uttar Pradesh 201306, India.
3
Govt.P.G.College Uttarkashi, Dist: Uttarkashi, Uttarakhnad, India.
*Corresponding author:
Dr. Rupa Mazumder, Professor, Dean (R&D) & HOD Pharmaceutics, Noida
Institute of Engineering and
Technology, Greater Noida, Uttar Pradesh 201306, India.
E-mail: rupa_mazumder@rediffmail.com, Tel: +91 9871963644.
ABSTRACT
The objective of the study was to explore the antioxidant activity of
ethanol extract of roots of Asparagus racemosus by in vitro and ex vivo models to substantiate the folklore claim of the
traditional practitioners. The roots of the plant showed sufficient
antioxidant activity by in vitro (determination of total
antioxidant activity through estimation of the conjugated dienes and
thiobarbituric acid reactive substances formed, total phenolic content,
reducing power, free radical scavenging activity by DPPH method, nitric
oxide scavenging activity, hydroxyl radical scavenging activity and super
oxide scavenging activity by NBT and DMSO methods) and ex vivo
(inhibition of haemolysis of RBC induced by phenyl hydrazine, inhibition of
lipid peroxidation induced by ferrous sulphate and carbon tetrachloride)
models. The experimental data were compared with that of standard
antioxidant like ascorbic acid, alpha-tocopherol acetate, butylated
hydroxyl anisol (BHA) and butylated hydroxyl toluene (BHT). All observed
results indicated ethanol extract of roots of Asparagus racemosus
to possess antioxidant activity in a concentration dependant manner and the
activity of the extract was found to be very much comparable to that of the
selected standard drugs.
Keywords:
Asparagus racemosus
, Antioxidant, Ethanol, Reactive oxygen species. Free
Radical Scavenging.