A high-performance liquid chromatographic method for determination of Valacyclovir in Pharmaceutical Dosage Forms and Rat Plasma

1 Suddhasattya Dey*, 1Anjan De, 1Arindam Sarkar, 2Prasana Pradhan, 2Shreya Shah, 2 Parmar Hardhik and 2Jayesh Thakar

1 Dr. B.C. Roy College of Pharmacy and Allied Health Sciences, Bidhan Nagar, Durgapur-713206, West Bengal, India.

2 Sigma Institute of Pharmacy, At-Bakrol, Woghodia, Near Ajwa-Nimata Road, Vadodara, Gujarat-390016, India.

Corresponding Author: kuntal.kuni@gmail.com


A simple, high performance liquid chromatographic method has been developed for the determination of valacyclovir in pharmaceutical dosage forms and rat plasma. The elution was performed using different mobile phase mixture of acetonitrile: methanol in ratio of 15:85 for pharmaceutical dosage form and acetonitrile: methanol: water in the ratio of 12:44:44 for plasma samples at a flow rate of 1.2 ml min-1 on a Phenomenex C18 column (150 × 4.6 mm, i.d., 5μm) at ambient temperature. The drugs were monitored at a wavelength of 260 nm and were separated within 10 min. Marketed formulations were prepared in suitable dilutions and plasma samples were prepared by precipitating proteins with the help of 25% perchloric acid. The method was successful in detecting the drugs at a concentration of less than 0.05 μg/ml. %RSD for intra- and inter-day studies was found to be within 8.83% for all the selected concentrations. Moreover, the method was validated as per ICH guidelines and the results were found to be within the acceptable range. Hence, the proposed method can be used for the routine quality control of the drugs and can also be applied to pharmacokinetic studies.

Keywords: Valacyclovir, Reverse phase HPLC, Validation, Rat plasma

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