Analytical Method Development For Simultaneous Determination Of Glucosamine Sulphate And Diacerein From A Mixture Using Derivative And Derivative Ratio Spectroscopy

Helen Chattopadhyaya, Sanhita Basu Mallickb , Sriparna Datta *.

a Research Scholar, Dept. of Chemical Technology, 92, A.P.C. Road, University of Calcutta, Kolkata,India.

b M.Tech final year student, Dept. of Chemical Technology, 92, A.P.C. Road, University of Calcutta, Kolkata,India.

*Associate Professor, Dept. of Chemical Technology, 92,A.P.C.Road, University of Calcutta, Kolkata ,India.

Corresponding Author:


The aim of the present work is to develop easy, fast and sensitive analytical method for the analysis of simultaneous estimation of Glucosamine sulphate (GS) & Diacerein(DC). The different concentration of GS & DC in the range of 8-40µg/mL and 2.5-20µg/mL respectively were analyzed. First derivative spectra of the mixtures containing different ratio of both the drugs were measured at 433.0 nm & 401.50 nm for GS and DC respectively. The concentration of the drugs was calculated from their corresponding regression equations measuring the intensity of the signals of the derivative ratio spectrum at 299.0 nm for GS and at 430.0 nm for DC. Limit of detection and limit of quantification for GS and DC were tested in triplicate which was found experimentally detectable. The average percent recovery was 100.58-99.46% for DC and 101.02-97.89% for GS. Percent relative standard deviation values of the methods were found within the acceptable range for both the drugs. It was observed that the deviation of the mean initial absorbance was less than 2.64% for analytical solution stability study which was within the acceptable range (±3%) indicating the stability of the analytical solutions. These methods were validated as per ICH guideline. Hence the proposed method can be used for the routine analysis of the drugs in a mixture.

Keywords: Glucosamine sulphate, Diacerein, Derivative spectrophotometry, Derivative Ratio Spectrophotometry, Method validation.

Journal Resources

Open Source


Abstracting and Indexing

Social Media