Analytical Method Development For Simultaneous Determination Of
Glucosamine Sulphate And Diacerein From A Mixture Using Derivative And
Derivative Ratio Spectroscopy
Helen Chattopadhyaya, Sanhita Basu Mallickb
, Sriparna Datta
*.
a
Research Scholar, Dept. of Chemical Technology, 92, A.P.C. Road, University
of Calcutta, Kolkata,India.
b
M.Tech final year student, Dept. of Chemical Technology, 92, A.P.C. Road,
University of Calcutta, Kolkata,India.
*Associate Professor, Dept. of Chemical Technology, 92,A.P.C.Road,
University of Calcutta, Kolkata ,India.
Corresponding Author:
sriparnadatta2014@gmail.com
ABSTRACT
The aim of the present work is to develop easy, fast and sensitive
analytical method for the analysis of simultaneous estimation of
Glucosamine sulphate (GS) & Diacerein(DC). The different concentration
of GS & DC in the range of 8-40µg/mL and 2.5-20µg/mL respectively were
analyzed. First derivative spectra of the mixtures containing different
ratio of both the drugs were measured at 433.0 nm & 401.50 nm for GS
and DC respectively. The concentration of the drugs was calculated from
their corresponding regression equations measuring the intensity of the
signals of the derivative ratio spectrum at 299.0 nm for GS and at 430.0 nm
for DC. Limit of detection and limit of quantification for
GS and DC were tested in triplicate which was found experimentally
detectable. The average percent recovery was 100.58-99.46% for DC and
101.02-97.89% for GS. Percent relative standard deviation values of the
methods were found within the acceptable range for both the drugs. It was
observed that the deviation of the mean initial absorbance was less than
2.64% for analytical solution stability study which was within the
acceptable range (±3%) indicating the stability of the analytical
solutions. These methods were validated as per ICH guideline. Hence the
proposed method can be used for the routine analysis of the drugs in a
mixture.
Keywords:
Glucosamine sulphate, Diacerein, Derivative spectrophotometry, Derivative
Ratio Spectrophotometry, Method validation.